ABC News reported Trump declined to enter a plea in person. Instead, attorney Todd Blanche entered the not-guilty plea on his behalf.
Blanche appeared before U.S. District Judge Shaniek Mills Maynard during the brief hearing.
Trump had already pleaded not guilty to dozens of other charges in the case.
New charges were brought against the former president and leading 2024 GOP contender and his longtime aide Walt Nauta as part of a superseding indictment introduced two weeks ago.